Mutations in components of the Wnt signaling pathway in gastric cancer

Figure 1 Typical elution profiles of DHPLC analysis and sequencing. A: AXIN1 (exon2) mutation. a, DHPLC elution profiles. b, Sequence traces. The upper panel is a normal control; the lower panel is a frameshift mutation (del 1 bp). The arrow points to the mutant nucleotide; B: AXIN1 polymorphism. c, Representative DHPLC profiles of heteroduplex for AXIN1. The lower panel is a normal control; the upper panel is a heteroduplex. d, Sequence traces. The arrow points to the mutant nucleotide; C: AXIN2 (exon7) mutation. e, DHPLC elution profiles. f, Sequence traces. The upper panel is a normal control; the lower panel is a frameshift mutation (del 1 bp). The arrow points to the mutant nucleotide; D: CTNNB1 (exon3) mutation. g, DHPLC elution profiles. The lower panel is a normal control; the upper panel is a frameshift mutation (del 27 bp spanning exon3). h, Sequence traces for the frameshift mutation. The arrow points to the mutant nucleotide.

Figure 2 Mutations and polymorphisms found in AXIN1. Bold lines indicate the coding regions corresponding to each protein-binding site. GSK3β: Glycogen synthase kinase 3β; PP2Ac: Protein phosphatase 2Ac; P: Polymorphism.