Adherence and invasion of mouse-adapted H pylori in different epithelial cell lines

Figure 1 Levels of adherence and invasion of H pylori in different cell lines. Bacteria were added to AGS cell monolayer at an MOI of 100 for 5 h at 37°C in a humidified atmosphere with 5% CO₂. Data were obtained by adherence and invasiveness assays in three independent experiments and are expressed as CFU per well of AGS cells. The left side 3 refrangible lines indicate the ability of the adherence in 3 different cell lines. The right sides indicate the invasiveness ability in the 3 cell lines. Values represent the mean CFU of viable bacteria recovered per well of a 24-well tissue culture tray.

Figure 2 Images showing morphologic changes induced by H pylori attachment. After co-culture of the bacteria with different cells for 5 h, the monolayer AGS cells were washed with PBS and the images were captured by microscopic Olympus BX51. A: AGS cell; B: The hummingbird/scattering appearance of AGS after being attached with 88-3887 wild-type, 5 h; C: AGS with H pylori 88-3887 cagA minus mutant (88-3887 cagA::cam), 5 h.