Analysis of the expression of coxsackievirus and adenovirus receptor in five colon cancer cell lines

Figure 1 A: Multiplex RT-PCR of expression of coxsackievirus and adenovirus receptor, PGM and β-actin (internal controls); B: CAR mRNA level in different cell lines. 1-6 refer to HEK (positive control), HT-29 (colon), LS180 (colon), SW480 (colon), SW948 (colon) and SW1116 (colon) cells, respectively.

Figure 2 Expression of CAR in colon cancer cell lines. Two micrograms of total RNA prepared from several different human colon cancer cell lines were subjected to RT-PCR analysis. RT-PCR revealed β-transcript expression in addition to the normal CAR gene transcripts.

Figure 3 Western blot analysis of CAR protein in colon cancer cell lines. Total protein from five human colon cancer cell lines was separated by SDS-PAGE, transferred to nitrocellulose membranes, and reacted with the correct anti-CAR antibodies.

Figure 4 Sensitivity of colon cancer cells to adenovirus infection. Cells were plated in 24-well culture plates and infected with Ad5-GFP at an MOI of 50. After 36 h, cells were evaluated for GFP expression by fluorescence microscopy. GFP was expressed when recombinant adenoviruses infected the cells.