Analysis of immune responses against H pylori in rabbits

Figure 1 Kinetics of anti H pylori antibody responses in rabbits. Female rabbits were infected with formalin-fixed H pylori whole cell antigens and blood samples were collected at various time points after H pylori infection. Sera (1:50 dilution) were subjected to ELISA for antibody titer measurement (in terms of OD). The arrows indicate the time points when rabbits were challenged with antigens (booster dose).

Figure 2 Western blot with whole cell extracts of H pylori against previously immunized rabbit sera and a human serum. Lane 1: molecular weight marker; lanes 2 to 8: serum collected on d 0, 7, 14, 21, 28, 35, and 42 respectively; lane 9: positive human serum.