Induction of ischemic tolerance in rat liver via reduced nicotinamide adenine dinucleotide phosphate oxidase in Kupffer cells

Figure 1 Viability of hepatocytes after anoxia/reoxygenation. (●): Viability of hepatocytes cultured under normoxia consistently. Viability of hepatocytes, pretreated with (○): 0 mmol/L; (▼): 0.03 mmol/L; ( ): 0.1 mmol/L; (■): 0.3 mmol/L;

Figure 2 ALT and LDH activities in perfusate after ischemia/reperfusion in an isolated perfused liver system from no preconditioning control, H₂O₂ preconditioning, GdCl₃-no preconditioning, and GdCl₃/H₂O₂ preconditioning rats. Columns and bars represent means ± SE from six rats in each group. ^bP < 0.01 vs no preconditioning, ^cP < 0.05 vs vehicle treatment and H₂O₂ preconditioning. KU: Karmen unit; WU: Wróblewski unit.

Figure 3 Oxygen radical formation in Kupffer cells after H₂O₂ preconditioning. A: Non-preconditioned livers; B: Livers after 10 min of H₂O₂ perfusion; C: Pretreatment with GdCl₃ and H₂O₂ perfusion; D: Treatment with DPI and H₂O₂ perfusion; E: The number of formazan-positive cells. Columns and bars represent means ± SE from 3 rats in each group. ^bP < 0.01 vs non-preconditioned control; ^dP < 0.01 vs H₂O₂ perfusion.

Figure 4 ALT and LDH activities in perfusate after ischemia/reperfusion in an isolated perfused liver system from no preconditioning control, H₂O₂ preconditioning, DPI-no preconditioning, and DPI/H₂O₂ preconditioning rats. Columns and bars represent means ± SE from eight rats in each group. ^bP < 0.01 vs no preconditioning; ^dP < 0.01 vs vehicle treatment and H₂O₂ preconditioning.

Figure 5 ALT and LDH activity in perfusate after ischemia/reperfusion in an isolated perfused liver system from no preconditioning control, ischemic preconditioning, DPI-no preconditioning, and DPI-ischemic preconditioning rats. Columns and bars represent means ± SE from six rats in each group. ^bP < 0.01 vs no preconditioning; ^cP < 0.05 vs vehicle treatment and ischemic preconditioning.