Hepatitis C Virus non-structural 5A abrogates signal transducer and activator of transcription-1 nucleartranslocation induced by IFN-α through dephosphorylation

Figure 1 HCV NS5A protein staining of Huh7 cells after 48 h transfection (DAB, × 400). A: P^CNS5A-transfected cells; B: P^RC/CMV-transfected cells; C: Non-transfected cells.

Figure 2 Tyr701 phosphorylated STAT1 in Huh7 cells induced by IFNα-2b (10 000 U/mL) (Western blot).

Figure 3 STAT1 or Phosphorylated STAT1 (Tyr701) in Huh7 cells stimulated by IFNα-2b (10 000 U/mL) for 30 min (FITC, × 400). A: STAT1; B: Tyr701.

Figure 4 STAT1 or phosphorylated STAT1 in cytoplasm and nuclei of Huh7 cells after 30 min IFN induced (FITC, × 400): A and D: P^CNS5A-transfected; B and E: P^RC/CMV-transfected; C and F: non-transfected.

Figure 5 Phosphorylated STAT1 from IFN induced Huh7 cells (FITC, × 400): A: P^CNS5A-transfected; B: P^RC/CMV-transfected; C: Non-transfected.