Detection of let-7a microRNA by real-time PCR in gastric carcinoma

Figure 1 Sensitivity and dynamic range of let-7a miRNA assay. A: Amplification curve of the synthesized let-7a miRNA, the well-behaved assay exhibited high sensitivity and broad dynamic range between the signal of 10 copies and 10⁸ copies input template and the no-template-control background; B: Standard curve of the let-7a miRNA (r² = 0.996, slope = -2.92).

Figure 2 Total RNA sensitivity and dynamic range of let-7a miRNA assay. A: Amplification plot of total RNA from gastric mucosa at six orders of magnitude. The total RNA input ranged from 50 pg to 5 μg in per stem-loop RT-PCR reaction; B: Correlation of total RNA input with the threshold of cycle (Ct) values for let-7a miRNA assays.

Figure 3 Expression of let-7a miRNA in gastric cancer. Difference in let-7a miRNA expression between in tumor tissues (T) and the adjacent non-tumor tissues (N). The patient numbers correspond to those in Table 2. Compared to normal tissues, let-7a miRNAs expression in tumor tissue from 14 patients was significantly down-regulated.