Characterization of hepatic progenitors from human fetal liver using CD34 as a hepatic progenitor marker

Figure 1 Sorted cells stained with FITC labeled anti human AFP antibody and observed under fluorescent (Zeiss) microscope (× 400). Cells were observed to be positive for AFP and a thin rim of cytoplasm with a big nucleus.

Figure 2 The graph shown is representative of the three independent experiments. The activity was expressed in μg/mg protein.

Figure 3 A: The sorted cells on d 0 of culture. Cells were cultured in the long term. The cells are showing intact morphology (× 400); B: The cultured cells on 14th d. The cells are showing intact morphology (× 400); C: The cultured cells on 35th d. The cells are showing intact morphology (× 400).

Figure 4 Culture of CD34+ sorted primary cells.

Figure 5 Gene expression analysis in different days of cultures of hepatic progenitors.