Screening and identification of interacting proteins with hepatitis B virus core protein in leukocytes and cloning of new gene C1

Figure 1 pGBKT7- HBcAg cut by EcoR I and Pst I on 0. 9% agarose/EtBr gel. Lanes 1 and 3: Marker; lane 2: HBcAg fragment cut by EcoR I and Pst I.

Figure 2 Western blotting shows the expression of HBcAg in yeast. Lane 1 is negative control and lane 2 is HBV core protein.

Figure 3 Screening colonies cut by BglII on 0. 9% agarose/EtBr gel

Figure 4 The nucleotide sequence of C1 gene and relevent amino acid sequences.

Figure 5 C1 DNA fragment amplified by RT-PCR. Lane 1 is Marker and lane 2 is a 366 bp fragment of C1, amplified by RT-PCR.

Figure 6 Positive clones interactive with the HBcAg protein grew on media lacking leucine, tryptophan, histidine and adenine.