Trimetazidine: Is it a promising drug for use in steatotic grafts?

Figure 1 AST (A) and ALT (B) levels in flushing effluent after 24-h cold storage. UW: liver preserved in UW solution; UW+TMZ: liver preserved in UW with TMZ. ^aP < 0.05 vs UW.

Figure 2 AST (A) and ALT (B) levels in perfusate after 30, 60, 90, and 120 min of normothermic reperfusion. UW: liver preserved in UW solution; UW+TMZ: liver preserved in UW with TMZ. ^aP < 0.05 vs UW.

Figure 3 Histological change in steatotic and non-steatotic livers after 120 min of normothermic reperfusion. Non-steatotic (A) and steatotic (B) livers preserved in UW solution: Cell swelling and disintegration of hepatic architecture were more evident in steatotic livers. Non-steatotic (C) and steatotic (D) livers preserved in UW solution with TMZ: Hepatic morphology was better preserved in both types of the liver compared to that recorded in UW solution.

Figure 4 Bile output (A) and percentage of sulfobromophthalein (BSP) in bile (B) of steatotic and non-steatotic livers after 120 min of normothermic reperfusion. UW: livers were preserved in UW solution; UW+TMZ: liver preserved in UW with TMZ. ^aP < 0.05 vs UW.

Figure 5 Hepatic malondialdehyde (MDA) levels after 120 min of normothermic reperfusion. UW: liver preserved in UW solution; UW+TMZ: liver preserved in UW with TMZ. ^aP < 0.05 vs UW.

Figure 6 Vascular resistance (A) and perfusion flow rate (B) of steatotic and non-steatotic livers after 120 min of normothermic reperfusion. UW: livers were preserved in UW solution; UW+TMZ: liver preserved in UW with TMZ. ^aP < 0.05 vs UW.

Figure 7 ATP (A) and adenine nucleotide (B) levels of steatotic and non-steatotic livers after 120 min of normothermic reperfusion. UW: livers were preserved in UW solution; UW+TMZ: liver preserved in UW with TMZ. ^aP < 0.05 vs UW.

Figure 8 Electron microscopy analysis of structural integrity of the mitochondria. Non-steatotic (A) and steatotic (B) livers preserved in UW solution: mitochondria swelling and the alterations in the electron density of their matrices were more evident in steatotic livers. Cristae are only seldom visible in steatotic livers. Non-steatotic (C) and steatotic (D) livers preserved in UW solution with TMZ. Mitochondrial integrity was better preserved in both types of liver compared to that recorded in UW solution.