Identification of differentially expressed genes in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis

Figure 1 The effect of RsaIdigestion. Lane 1, 3: cDNA of Hca-F and Hca-P cells; Lane 2, 4: cDNA of Hca-F and Hca-P cells after RsaIdigestion; M: DNA marker DL2000.

Figure 2 Ligation efficiency analysis. Lane 1: Tester-1 as template, G3PDH 3’Primer and PCR Primer1; Lane 2: Tester-1 as template, G3PDH 3‘Primer and G3PDH 5‘Primer; Lane 3: Tester-2R as template, G3PDH 3‘Primer and PCR Primer1; Lane 4: Tester-2R as template, G3PDH 3‘Primer and G3PDH 5‘Primer.

Figure 3 The results of secondary PCR amplification. Lane 1-3: Product of primary PCR amplification, Lane 4: secondary PCR amplification product of unsubtracted cDNA, Lane 5: secondary PCR amplification product of subtracted cDNA, Lane 6: secondary PCR amplification product of PCR control cDNA, M: DNA Marker DL2000.

Figure 4 Analysis of subtraction effect. PCR was performed on subtracted (Lane 1-4) or unsubtracted (Lane 5-8) secondary PCR product with G3PDH 5’Primer and 3’primer. Lanes 1, 5: 20 cycles, Lanes 2, 6: 25 cycles, Lanes 3, 7: 30 cycles, Lanes 4, 8: 35 cycles. M: DNA marker DL2000.

Figure 5 The results of clone PCR amplification. There was an average insert size of 300-1000 bp. M: DNA marker DL2000.