Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases

Figure 1 Expression of chemokine/chemokine receptor pairs in HCC and CRLM as determined by Q-RT-PCR. A: CXCL12/CXCR4 expression; B: CCL20/CCR6 expression; C: CCL19/CCL21/CCR7 expression (mean ± SE, ^aP < 0.05, n = 11 and 23, respectively).

Figure 2 Expression of CXCL12, CCL20, CCL19 and CCL21 in HCC and CRLM as determined by the enzyme-linked immunosorbant assay (ELISA). A: Chemokine expression in HCC; B: Chemokine expression in CRLM. (ng/L chemokine ligand related to 1 g total protein for the HCC and CRLM tissues. mean ± SE, ^aP < 0.05; n = 11 and 23, respectively).

Figure 3 Expression of chemokine receptor CCR6 in HCC and CRLM as determined by Western blot analysis. A: Chemokine expression in HCC; B: Chemokine expression in CRLM. Total cell lysates of tumor (P) and corresponding normal tissues (N) of three patients with HCC and CRLM, respectively, were immunoblotted with antibodies specifically recognizing chemokine receptor CCR6. Cell line HL60 served as a positive control for the detection of CCR6. (n = 11 and 23, respectively).