BRAF, K-ras and BAT26 mutations in colorectal polyps and stool

Figure 1 Example of BRAF mutation analysis in adenoma tissues (T) and paired stool (S) samples. A: SSCP analysis; B: Direct sequencing showing the wild type BRAF; C: BRAF V599E mutation; N: normal. Arrows indicate the new band (A) and the mutation site (C).

Figure 2 Analysis of K-ras mutations at codon 12 (A) and codon 13 (B) in adenoma tissues (T) and paired stool (S) samples. Fragments of 157 bp indicate mutations, and fragments of 128 bp(A) or 125 bp (B) respectively represent wild-type alleles. M: 100 bp ladder marker; P: Positive control; B shows the result that a mutation at codon 13 of K-ras in stool DNA was not observed in tumor counterpart.