Correlative studies on uPA mRNA and uPAR mRNA expression with vascular endothelial growth factor, microvessel density, progression and survival time of patients with gastric cancer

Figure 1 In situ hybridization of uPA mRNA in gastric cancer tissue. A: Negative in the plasma of non-tumor gastric mucosa (ISH × 100); B: Positive in the plasma of gastric adenocarcinoma (ISH × 220); C: Positive in plasma of gastric adenocarcinoma with lymphangial cancer embolus (ISH × 180); D: Positive in the plasma of gastric adenocarcinoma with greater peritoneum infiltration (ISH × 180).

Figure 2 In situ hybridization of uPAR mRNA in gastric cancer tissue. A: Negative in the plasma of non-tumor gastric mucosa (ISH × 100); B: Positive in the plasma of gastric adenocarcinoma infiltrated to muscularis (ISH × 180); C: Positive in plasma of gastric adenocarcinoma with lymphangial cancer embolus (ISH × 240); D: Positive in the plasma of gastric adenocarcinoma with greater peritoneum infiltration (ISH × 120).

Figure 3 VEGF expression in gastric cancer tissue. A: Negative in the plasma of non-tumor gastric mucosa (SP×220); B: Positive in the plasma of gastric adenocarcinoma with greater omentum infiltration (SP × 220); C: Positive in plasma of gastric adenocarcinoma at the front of the cancer infiltration areas (SP × 180).

Figure 4 Microvessels in gastric cancer. A: Negative CD34 in the vascular endothelial cell of non-tumor gastric mucosa (SP × 120); B: Positive CD34 in the vascular endothelial cell of gastric adenocarcinoma with MVD < 54.9 (SP × 400); C: Positive of CD34 in the vascular endothelial cell of gastric adenocarcinoma with MVD ≥ 54.9 (SP × 120).

Figure 5 Keplan-Meier survival curves of gastric adenocarcinoma. A: With or without uPA mRNA (P < 0.05); B: With or without uPAR mRNA (P < 0.05); C: With or without VEGF (P < 0.05); D: With MVD ≥ 54.9 and MVD < 54.9 (P < 0.05).