Efficient expression of histidine-tagged large hepatitis delta antigen in baculovirus-transduced baby hamster kidney cells

Figure 1 Schematic illustration of recombinant donor plasmids pBac-GDH and pBac-GD

Figure 2 Western blot analysis of proteins expressed by mock-, Bac-GD- or Bac-GDH-transduced BHK cells. A: Anti-HDV sera; B: Anti-His₆ mAb.

Figure 3 A: Variation of the transduction efficiency (TE) over time post-transduction. B: Time-course profile of L-HDAgH expression. Western blot using anti-HDV sera and anti-His6 mAb.

Figure 4 Effects of sodium butyrate on L-HDAgH expression. A. Western blot; B. The expression levels.

Figure 5 Subcellular localization and isoprenylation of L-HDAgH by immunofluorescence labeling. A: the presence of nuclei labeled by SYBR green I; B: L-HDAg detected using Cy3-conjugated goat anti-mouse IgG; C: merged photograph of A and B; D: the presence of L-HDAgH labeled by FITC-conjugated mAb; E: The presence of isoprenylation marked by TRITC-conjugated mAb; F:merged photograph of D and E. Bars, 20 μm.

Figure 6 Western blot using anti-HBsAg, anti-HDAg and anti-His₆ antibodies.

Figure 7 Western blot (A) and SDS-PAGE(B). M, marker; ori, original sample; fl, flow through; R, samples stuck in the resin. The numbers indicate the imidazole concentration at which the sample was collected.