Alcohol dehydrogenase: A potential new marker for diagnosis of intestinal ischemia using rat as a model

Figure 1 Alcohol Dehydrogenase Assay By Skursky et al (A) and SGPT Assay (B) performed in heart blood and portal blood of 1 h ischemic test and sham operated control group. A: Alcohol dehydrogenase assay performed by Skursky et al method. CH and TH represent the blood withdrawn from heart of control group and test group, respectively. CP and TP represent the blood withdrawn from portal vein of control group and test group, respectively. DAC and DAT represent the dorsal aorta blood sample from control and test group, respectively while IVCC and IVCT represent the blood sample from inferior vena cava of control and test group, respectively. The EU expressed in terms of micromoles of NDMA reduced per min/L of serum; B: SGPT assay. CH and TH represent the blood withdrawn from heart of control group and 1 h ischemic test group, respectively. CP and TP represent the blood withdrawn from portal vein of control group and 1 h ischemic test group, respectively.

Figure 2 Kinetic assay of Alcohol dehydrogenase from serum carried out at 30 °C. (B) Showing practically no auto-reduction of NDMA in the absence of serum, (C) reduction of NDMA in the presence of serum drawn from the heart of control animal after 1 h of sham operation, (T) reduction of NDMA in presence of serum drawn from the heart of test animal after 1 h SMA occlusion.

Figure 3 Histology of intestine from rats. A: C- control sham operated group; 0 grade injury i.e. no damage to villi and crypts; B: t-test one-hour intestinal ischemic group; Total villous loss with some crypts involved; stroma normal. Vessels are patent (i.e. grade 3 injury).