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©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Feb 14, 2005; 11(6): 831-838
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.831
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.831
Figure 1 Schematic outline of siRNA expressing adenovirus AdH1-K-rasval12, AdH1-p53 and AdH1-empty.
Figure 2 Foci produced by adenoviruses on d 2-7 and 12 after transfection of 293 cells.
Figure 3 Sequence (for detail, refer to[46]) of Val12 mutant alleles of human K-ras and the predicted mutant-specific short hairpin transcripts encoded by AdH1-K-rasval12.
Figure 4 Representative Western blot analysis for K-rasval12 expression in Panc-1 and HeLa cells 60 h after infection with viral stocks (AdH1-K-rasval12, AdH1-empty, AdH1-p53) of three separate experiments.
Figure 5 Northern blot analysis with total RNA from same infected Panc-1 cells of single experiment.
Figure 6 Apoptotic indices shown as the mean of four separate experiments for each group (P<0.
05).
Figure 7 Stable polyclonal pools of Panc-1 and HeLa cells harboring wild-type H-ras infected with the indicated viral stocks and immunoblotted for the detection of p53 and actin proteins in single experiment.
Figure 8 Detection of K-rasval12 at protein level in Panc-1 and HeLa cells without virus infection in single experiment.
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Citation: Chen LM, Le HY, Qin RY, Kumar M, Du ZY, Xia RJ, Deng J. Reversal of the phenotype by
K-rasval12 silencing mediated by adenovirus-delivered siRNA in human pancreatic cancer cell line Panc-1. World J Gastroenterol 2005; 11(6): 831-838 - URL: https://www.wjgnet.com/1007-9327/full/v11/i6/831.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i6.831