Copyright
©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Feb 14, 2005; 11(6): 785-790
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.785
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.785
Figure 1 Inhibition of telomerase activity by telomerase ASODN.
A: The relative telomerase activity (% control) of SW480 cells transfected with 0.2 µmol/L ASOND for 24, 48, 72 h respectively. Extracts of SW480 cell were quantified with a TRAPEZE® ELISA telomerase detection kit. Sensitivity of telomerase PCR-ELISA (mean±SD of three experiments). B: Telomerase activity (% control) of SW480 cells transfected by ASODN with various concentrations for 72 h. The concentration of ASODN was 0.05, 0.1, 0.2 µmol/L, respectively (mean±SD of three experiments). C: PAGE figure of TRAP-PCR: Lane 1, Oligofectamine; lane 2, S-hTR; lane 3, anti-hTR; lane 4, anti-hTERT; lane 5: S-hTERT; lane 6, control; lane 7, anti-hTR+anti-hTERT; lane 8, negative; ITAS was the 36-bp internal control. Negative was lysis buffer.
Figure 2 The inhibition on proliferation of SW480 cells transfected with 0.
2 µmol/L ASODN. A: Cells were exposed to various agents for 24, 48, 72 h, respectively; B: Cells were exposed to various concentrations of ASODN for 72 h (mean±SD of three experiments).
Figure 3 Apoptosis induced by treatment with ASODN in SW480 cells.
A: Cells were transfected with various agents (1: blank control, 2: s-hTR, 3: s-hTERT, 4: OligofectamineTM, 5: anti-hTR, 6: anti-hTERT, 7: anti-hTR+anti-hTERT) for 72 h; B: Cells were transfected with ASODN for 24, 48, 72 h, respectively (mean±SD of three experiments).
- Citation: Fu XH, Zhang JS, Zhang N, Zhang YD. Combination of telomerase antisense oligonucleotides simultaneously targeting hTR and hTERT produces synergism of inhibition of telomerase activity and growth in human colon cancer cell line. World J Gastroenterol 2005; 11(6): 785-790
- URL: https://www.wjgnet.com/1007-9327/full/v11/i6/785.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i6.785