Disrupted NF-κB activation after partial hepatectomy does not impair hepatocyte proliferation in rats

Figure 1 Effect of saline, PDTC, and PTX on NF-κB binding activity. A: Nuclear extracts were prepared from non-treated rats (control), from rats injected with saline, PDTC or PTX subjected or not to partial hepatectomy (PH). No NF-κB DNA binding activity was observed in the liver nuclear extracts of non-treated rats (controls) nor 1 h after injection of PDTC or PTX. Faint DNA binding was noticed 1 h after the injection of saline which mainly corresponds to NF-κB p50. Thirty minutes after PH, strong DNA binding was observed in saline-treated groups and two complexes were identified. Supershift experiments identified the upper complex in saline +PH as p65/p50. In the PTX group, a third complex was present 0.5 h after PH, which migrated faster than the dose obtained after saline; B: Detailed supershift experiments showed that this faster migrating complex in the PTX+PH group was completely supershifted with the p65 antibody. p52, c-rel and rel-B had no influence on the NF-κB DNA binding activity.

Figure 2 Thymidine incorporation. Similar thymidine incorporation was obtained 24 h after PH in rats pre-treated with an injection of PDTC or PTX compared to non-treated partial hepatectomized rats.

Figure 3 PCNA labeling index. Similar PCNA labeling index was obtained 24 h after PH in rats pre-treated with an injection of PDTC, or PTX compared to non-treated partial hepatectomized rats.

Figure 4 Effect of saline, PDTC and PTX on Stat3 DNA binding activity. No Stat3 DNA binding activity was identified in controls (C). Injection of saline, PDTC or PTX enhanced the binding at 1 h. At 0.5 h after PH, strong Stat3 DNA binding was observed in rats injected with saline, PDTC or PTX. Supershift experiments with a Stat3 specific antibody confirmed that Stat3 is the principal compound of these DNA-binding complexes.