Effects of ethanol on antioxidant capacity in isolated rat hepatocytes

Figure 1 Lactate dehydrogenase (LDH) leakage of primary rat hepatocytes in each group. Data are mean±SD for 3 hepatocyte preparations. Cultures were incubated without ethanol (C), with 10 mmol/L ethanol (E 10), with 50 mmol/L ethanol (E 50), and with 100 mmol/L ethanol (E 100) for 12, 24, and 36 h, respectively. ^aP<0.05 vs 24 h of group C and E 10; ^cP<0.05 vs 36 h of groups C and E 10.

Figure 2 Lipid peroxidation of primary rat hepatocytes in each group. Lipid peroxidation was estimated by the measurement of MDA. Data are mean±SD for 3 hepatocyte preparations. Cultures were incubated without ethanol (C), with 10 mmol/L ethanol (E 10), with 50 mmol/L ethanol (E 50), and with 100 mmol/L ethanol (E 100) for 12, 24, and 36 h, respectively. ^aP<0.05 vs 36 h of groups C, E 10 and E 50; ^cP<0.05 vs 12 h of group E 50; ^eP<0.05 vs 12 h and 24 h of group E 100.

Figure 3 Glutathione (GSH) concentrations of primary rat hepatocytes in each group. Data are mean±SD for 3 hepatocyte preparations. Cultures were incubated without ethanol (C), with 10 mmol/L ethanol (E 10), with 50 mmol/L ethanol (E 50), and with 100 mmol/L ethanol (E 100) for 12, 24, and 36 h, respectively. ^aP<0.05 vs 12 h of group E 100.

Figure 4 Glutathione peroxidase (GPX) activities of primary rat hepatocytes in each group. Data are mean±SD for 3 hepatocyte preparations. Cultures were incubated without ethanol (C), with 10 mmol/L ethanol (E 10), with 50 mmol/L ethanol (E 50), and with 100 mmol/L ethanol (E 100) for 12, 24, and 36 h, respectively. ^aP<0.05 vs 12 h of group E10; ^cP<0.05 vs 12 h of group E 50; ^eP<0.05 vs 12 h of group E 100.

Figure 5 Glutathione reductase (GRD) activities of primary rat hepatocytes in each group. Data are mean±SD for 3 hepatocyte preparations. Cultures were incubated without ethanol (C), with 10 mmol/L ethanol (E 10), with 50 mmol/L ethanol (E 50), and with 100 mmol/L ethanol (E 100) for 12, 24, and 36 h, respectively. ^aP<0.05 vs 36 h of groups C and E 10; ^cP<0.05 vs 12 h and 24 h of groups C; ^eP<0.05 vs 12 h and 24 h of group E 10.

Figure 6 Superoxide dismutase (SOD) activities of primary rat hepatocytes in each group. Data are mean±SD for 3 hepatocyte preparations. Cultures were incubated without ethanol (C), with 10 mmol/L ethanol (E 10), with 50 mmol/L ethanol (E 50), and with 100 mmol/L ethanol (E 100) for 12, 24, and 36 h, respectively. ^aP<0.05 vs 12 h of group E50; ^cP<0.05 vs 12 h of group E 100.

Figure 7 Catalase (CAT) activities of primary rat hepatocytes in each group. Data are mean±SD for 3 hepatocyte preparations. Cultures were incubated without ethanol (C), with 10 mmol/L ethanol (E 10), with 50 mmol/L ethanol (E 50), and with 100 mmol/L ethanol (E 100) for 12, 24, and 36 h, respectively. ^aP<0.05 vs 12 h of groups C; ^cP<0.05 vs 36 h of groups C and E 50; ^eP<0.05 vs 12 h of group E 50; ^gP<0.05 vs 12 h of group E 100.