Hepatitis C virus non-structural 5A protein can enhance full-length core protein-induced nuclear factor-κB activation

Figure 1 Expression of core mutant fragments (A) and NS5A protein (B).

Figure 2 Effect of HCV core and NS5A proteins on NF-κB activity. A: NF-κB activation capacity of the core mutant fragments and NS5A protein individually expressing in Huh7 cell line; B: NF-κB activation capacity of the core mutants and NS5A protein expressing in Huh7 cells, C: Cos-7 cells; D: HeLa cells.

Figure 3 Enhancement of core protein-induced NF-kB activation in a NS5A protein dose-dependent manner.

Figure 4 Expression of NF-κB/p65, NF-κB/p50 and IkB-a.

Figure 5 Localization of C191 and NS5A proteins. A-C: C191; D-F: NS5A protein; G: NS5A protein (FITC-labeled); H: C191 protein (TRITC-labeled); I: merged localization of C191 and NS5A proteins; J-L: pcDNA3 vector alone as a negative control.