Apoptosis induced by nucleosides in the human hepatoma HepG2

Figure 1 Effects of nucleosides on the cell cycle progression of HepG2 cells. HepG2 cells untreated (control) or treated with nucleosides were stained with propidium iodide and analyzed using a flow cytometer. The contents of inosine, cytidine, uridine, and thymidine in mediums were 30 mmol/L, respectively. And, the contents of adenosine and guanosine were 0.5 mmol/L. This experiment was repeated thrice and obtained similar results were obtained. Figure 1 was the example from all results in each group. M1, M2, M3, and M4 represented Sub G1, G0/G1, S, and G2/M phases, respectively (A-G).

Figure 2 Effects of nucleosides on the p53 and p21 protein expressions in HepG2 cells. One hundred μg of cytosolic proteins were separated by SDS-PAGE, and P53, P21, and β-actin protein were detected, respectively. This experiment was repeated thrice and similar results obtained.