Identification of anrF gene, a homology of admM of andrimid biosynthetic gene cluster related to the antagonistic activity of Enterobacter cloacae B8

Figure 1 Framework of the cloning of full-length anrF gene with transposon tagging and chromosome walking.

Figure 2 PCR products from three rounds of chromosomal walking. 1: PCR products, 1 209 bp in size, amplified from B8/PstI-cassette library with FS2 and CP primers; 2: PCR products, 962 bp in size, amplified from B8/Sau3AI cassette library with F1815 and CS; 3: PCR products, mainly 900 bp in size, amplified from B8/PstI cassette library with F2000 and CP; M: marker DL2000.

Figure 3 Alignment of nucleotide sequences of anrF gene and admM gene of P. agglomerans andrimid biosynthetic gene cluster (AY192157). The names of the genes are typed at the left and the number of nucleotides is at the right. The identical nucleotides between two genes are marked with gray background.

Figure 4 PCR amplification of full-length anrF gene. 1: PCR products amplified from B8; 2: PCR products amplified from B8F; M: marker 3 kb.

Figure 5 Antagonistic activity of B8, B8F and B8F/pMD-FG. B8F+: pMD-FG transformed B8F.