DNA ploidy analysis and expression of MMP-9, TIMP-2, and E-cadherin in gastric carcinoma

Figure 1 MMP-9 expression in gastric carcinoma. A: MMP-9 expression showed moderate or diffuse distribution of immunostaining in neoplastic cells (×200); B: single or focal distribution in noncancerous mucosa cells (×400); C: in the invasion front, MMP-9 showed strong positive staining (×200); D: positive staining was also noted in vascular endothelial cells and inflammatory cells (×400).

Figure 2 TIMP-2 expression in gastric carcinoma. A: TIMP-2 expression showed moderate or diffuse distribution in neoplastic cells (×200), B: focal or moderate distribution in noncancerous mucosa cells (×400).

Figure 3 E-cadherin expression in gastric carcinoma. A: E- cadherin expression showed strong membranous staining in noncancerous mucosa cells, especially at the intercellular border (×400); B-D: abnormal expression was observed in neoplastic tissues which showed reduction or loss of membranous expression and showed cytoplasmic or nuclear staining (B and D, ×200; C, ×400).

Figure 4 Histograms from FCM. A: A large diploid peak and a smaller tetraploid peak; B: an aneuploid peak (see arrow); C: higher SPF (see arrow).