Protective effects of non-mitogenic human acidic fibroblast growth factor on hydrogen peroxide-induced damage to cardiomyocytes in vitro

Figure 1 Cardiac myocytes identified by immunocytochemical assay.

Figure 2 Cellular morphological change of cardiomyocytes after exposure to different concentrations of hydrogen peroxide for 4 h. A: Normal control group (magnification ×200); B: cardiomyocytes exposure to 1.25 mmol/L H₂O₂ (magnification ×200); C: cardiomyocytes exposure to 2.5 mmol/L H₂O₂ (magnification ×200); D: cardiomyocytes exposure to 5 mmol/L H₂O₂ (magnification ×200).

Figure 3 Electrophoresis analysis of DNA extracted from cultured myocytes. Lane 1: normal control group; lane 2: 0.625 mmol/L H₂O₂ for 3 h; lane 3: 1.25 mmol/L H₂O₂ for 3 h; lane 4: 2.5 mmol/L H₂O₂ for 3 h; lane 5: 5 mmol/L H₂O₂ for 3 h; lane 6: 10 mmol/L H₂O₂ for 3 h; lane 7: DNA marker.

Figure 4 Anti-apoptosis effect of nm-haFGF on cardiomyocytes injured by H₂O₂.