Copyright
©The Author(s) 2005.
World J Gastroenterol. Aug 21, 2005; 11(31): 4812-4814
Published online Aug 21, 2005. doi: 10.3748/wjg.v11.i31.4812
Published online Aug 21, 2005. doi: 10.3748/wjg.v11.i31.4812
Figure 1 Identification of the recombinant clone of NS and its expression in yeast cell.
A: Analysis of pGBKT7-NS with restriction enzyme digestion. M: DNA markers; lane 1: undigested pGBKT7-NS; lane 2: digestion of pGBKT7-NS with BamHI/XhoI and 1 680-bp fragment was released; B: Western blotting analysis of NS expression by pGBKT7-NS in yeast. Total protein from yeast AH109 transferred with pGBKT7-NS was subjected to Western blot and the NS mAb was used to detect the NS protein. Lane 1: total proteins from pGBKT7-NS transferred AH109; lane 2: total proteins from pGBKT7 transferred AH109.
Figure 2 Restriction enzyme analysis of recombinant pcDNA3-NS and pcDNA3-myc-PPP2R5A.
M: DNA markers; lane 1: undigested pCDNA3-NS; lane 2: digestion of pCDNA3-NS by BamHI/XhoI and 1 680-bp fragment was released; lane 3: undigested pcDNA3-myc-PPP2R5A; lane 4: digestion of pcDNA3-myc-PPP2R5A with BamHI/XhoI and 2 300 bp fragment was released.
Figure 3 Co-immunoprecipitation of protein NS and PPP2R5A.
Total proteins from cells co-transferred with plasmids pCDNA3-NS and pcDNA3-myc-PPP2R5A were used for immunoprecipitation with antibody against NS or c-myc and antibody against c-myc or NS was used in Western blot. Mouse IgG was used in immunoprecipitation as negative control.
- Citation: Yang HX, Jin GL, Meng L, Zhang JZ, Liu WB, Shou CC. Screening and identification of proteins interacting with nucleostemin. World J Gastroenterol 2005; 11(31): 4812-4814
- URL: https://www.wjgnet.com/1007-9327/full/v11/i31/4812.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i31.4812