Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat

Figure 1 Effect of IL-10 on liver histology of hepatic fibrosis rats. A: Rat liver in group C at the 7^th wk (HE×100); B: Rat liver in group I at the 7^th wk (HE×100); C: Rat liver in group C at the 11^th wk (HE×100); D: Rat liver in group I at the 11^th wk (HE×100); E: Rat liver in group N (HE×100).

Figure 2 Desmin expression of HSCs from primary culture for 5 d by immunochemistry (SP×100).

Figure 3 RT-PCR analysis of growth factor gene expression of HSC before and after intervention. A: Electrophoretogram of PCR for TGF-β1 gene of HSCs. 1-3: groups N, C, I of 11^th wk; 4-6: groups N, C, I of 7^th wk; M: DNA ladder of 100 bp; B: Electrophoretogram of PCR for EGF gene of HSCs. 1-3: groups N, C, I of 11^th wk; 4-6: groups N, C, I of 7^th wk; M: DNA ladder of 100 bp; C: Electrophoretogram of PCR for HGF gene of HSCs. 1-3 groups N, C, I of 11^th wk; 4-6: groups N, C, I of 7^th wk; M: DNA ladder of 100 bp.

Figure 4 Expression of growth factor protein of HSCs in different groups. A: Expression of PDGF protein of HSCs in group C at the 7^th wk (SP×100); B: Expression of PDGF protein of HSCs in group I at the 7^th wk (SP×100); C: Expression of TGF-β1 protein of HSCs in group C at the 7^th wk (SP×100); D: Expression of TGF-β1 protein of HSCs in group I at the 7^th wk (SP×100); E: Expression of EGF protein of HSCs in group C at the 11^th wk (SP×100); F: Expression of EGF protein of HSCs in group I at the 11^th wk (SP×100).