Functional expression of a proliferation-related ligand in hepatocellular carcinoma and its implications for neovascularization

Figure 1 APRIL expression in human HCC cell lines and their supernatants by Western blotting. Closed arrowhead indicates the expression of unprocessed form of APRIL (42 ku) and open arrowhead indicates the soluble form of APRIL (17 ku).

Figure 2 Effects of rhAPRIL stimulation on viability of human HCC cells (A) and HUVECs (B and C) incubated at different time periods.

Figure 3 Regulation of APRIL expression in HCC cells. Closed arrowhead indicates the expression of unprocessed form of APRIL. Open arrowhead indicates soluble form of APRIL.

Figure 4 Regulation of VEGF expression with starvation in HCC cells incubated with FBS-negative culture (A) and 10 μg/mL anti-APRIL polyclonal antibody or normal rabbit IgG (B).

Figure 5 Effect of APRIL on in vitro endothelial cell tube formation.