Preparation of anti-mouse caspase-12 mRNA hammerhead ribozyme and identification of its activity in vitro

Figure 1 Agarose gel electrophoresis of caspase-12 RT-PCR product. Lane 1: DL 2000 DNA Marker; lane 2: The 866 bp caspase-12 gene segment produced by RT-PCR.

Figure 2 In vitro transcription product of caspase-12 segment, separated by 6% PAGE, the transcript was 910 nt.

Figure 3 Agarose gel electrophoresis of PCR amplified ribozyme templates. Lane 1: DL 2000 DNA Marker; lane 2: Rz138 template produced by PCR; lane 3: Rz218 template produced by PCR.

Figure 4 In vitro Cleavage of mouse caspase-12 mRNA by ribozymes. A: The length of caspase-12 segment transcribed from BamHI linearized pCaspase-12 which contains the mouse caspase-12 gene segment, and it’s anticipated cleavage products. B: The results of cleavage reaction by PAGE electrophoresis. Lane 1: Rz138 cleavage result; lane 2: Rz218 cleavage result; lane 3: control (control contains all other components except ribozyme.).