Effect of caffeic acid phenethyl ester on proliferation and apoptosis of colorectal cancer cells in vitro

Figure 1 Effect of CAPE on HCT116 cell proliferation.

Figure 2 Effect of CAPE on HCT116 cell apoptosis rates.

Figure 3 Annexin-V and PI double-staining FCM of HCT116 cells following 24 h incubation with (A) control, (B) 2. 5 mg/L CAPE, (C) 5.0 mg/L CAPE, (D)10 mg/L CAPE.

Figure 4 TUNEL assay of HCT116 cells following 24 h incubation with (A) 2. 5 mg/L CAPE, (B) 10 mg/L CAPE.

Figure 5 Flow cytometry of HCT116 cells following 24 h incubation with (A) control, (B) 2. 5 mg/L CAPE, (C) 5.0 mg/L CAPE, (D) 10 mg/L CAPE.

Figure 6 Western blot analysis of β-catenin protein expression in HCT116 cells following 24 h (Lanes 1-4) and 48 h (Lanes 5-8) incubation with different concentrations of CAPE. Lanes 1-4: control, 2.5 mg/L CAPE, 5.0 mg/L CAPE, 10 mg/L CAPE; Lanes 5-8: control, 2.5 mg/L CAPE, 5.0 mg/L CAPE, 10 mg/L CAPE.

Figure 7 Indirect immunofluorescence analysis of β-catenin protein expression in HCT116 cells following 48 h incubation with (A) control, (B) 10 mg/L CAPE.