Urinary nucleosides as biological markers for patients with colorectal cancer

Figure 1 Schematic diagram of the column-switching HPLC system.

Figure 2 Typical chromatograms of (A) 14 standard nucleoside mixtures (B) urinary nucleosides of a normal urine obtained under the established analysis conditions. Column-switching HPLC conditions as Table 2. Peak identification: 1 Pseu; 2 C; 3 U; 4 m1A; 5 I; 6 m5U; 7 G; 8 m1I; 9 m1G; 10 ac4C; 11 m2G; 12 A; 13 m22G; 14 Br8G; 15 m6A.

Figure 3 PCA based on 14 nucleoside concentrations from healthy controls (+) and patients with colorectal cancer (o). A: Positions of patients with intestinal villous adenoma (*) were marked into the figure based on classification equation from healthy controls and patients with colorectal cancer; B: Positions of 32 of responsive cases after surgery (*) were marked into the figure based on classification equation from healthy controls and patients with colorectal cancer; C: Positions of 11 of progressive cases after surgery (*) were marked into the figure based on classification equation from healthy controls and patients with colorectal cancer.