Molecular epidemiological study on pre-X region of hepatitis B virus and identification of hepatocyte proteins interacting with whole-X protein by yeast two-hybrid

Figure 1 Nucleotide sequence of the pre-X gene.

Figure 2 Expression of whole-X protein in yeast demonstrated by Western blotting. Lane 1: whole-X protein, lane 2: negative control.

Figure 3 Colonies cut by EcoRI on 0. 9% agarose/EtBr gel.

Figure 4 Positive clones interactive with the whole-X protein growing on media containing X-α-Gal and lacking leucine, tryptophan, histidine and adenine.