Copyright
©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Jun 7, 2005; 11(21): 3300-3303
Published online Jun 7, 2005. doi: 10.3748/wjg.v11.i21.3300
Published online Jun 7, 2005. doi: 10.3748/wjg.v11.i21.3300
Figure 1 scFv gene from SOE PCR amplification.
Lanes A and F: DNA markers; lanes B-E: SOE PCR products.
Figure 2 plasmid PCR products.
A: DNA marker; B and C: PCR products.
Figure 3 SDS-PAGE of ScFv induced products.
A: Negative bacteria induced by IPTG; B: low molecular mass protein markers; C: positive bacteria induced by IPTG.
Figure 4 ScFv- pDAP2 plasmid PCR.
M: DNA molecular markers; A-G: PCR products.
Figure 5 SDS-PAGE of scFv-AP expressing product.
A: Negative bacteria induced by IPTG; B: low molecular mass protein markers; C: positive bacteria induced by IPTG.
- Citation: Yang DF, Zhu HF, Wang ZH, Shen GX, Tian DY. Construction of single chain Fv antibody against transferrin receptor and its protein fusion with alkaline phosphatase. World J Gastroenterol 2005; 11(21): 3300-3303
- URL: https://www.wjgnet.com/1007-9327/full/v11/i21/3300.htm
- DOI: https://dx.doi.org/10.3748/wjg.v11.i21.3300