Drug sensitivity and drug resistance profiles of human intrahepatic cholangiocarcinoma cell lines

Figure 1 Expressions of TS, DPD, GSTP1, MDR1 and MRPs mRNA in five CCA cell lines determined by semi-quantitative RT-PCR analysis. Total RNA was extracted and used for RT-PCR. Each lane was loaded with 25 μL of PCR product. mRNA of the gene of interest was co-amplified with GAPDH mRNA as an internal control. Lane 1 is 100 bp DNA marker, lane 2-6 are PCR products from KKU-100, KKU-M156, KKU-OCA17, KKU-M055 and KKU-M214, respectively.

Figure 2 Relationships between the relative mRNA expression of MRP3 and the IC₅₀ value of etoposide. (A) doxorubicin; (B) and pirarubicin; (C) in five CCA cell lines.