Increased activity of Pgp multidrug transporter in patients with Helicobacter pylori infection

Figure 1 FACS analysis of Rh123 accumulation as a function of Pgp activity (A-C): (A) GBS were stained with a fluorescent substrate Rh123 in the presence (overlaid histogram) or absence (filled histogram) of the MDR reversing agent CyA. Pgp activity is expressed as RMF, a ratio of mean fluorescences obtained from the gated viable cell population. RMF ≥1 considered positive; (B) resistant (Pgp-positive/active) and (C) sensitive (Pgp-negative/inactive) control cell lines.

Figure 2 Enhanced Pgp activity in patients with H pylori infection. RMF values of 53 H pylori positive patients and 12 H pylori negative (control) patients are presented (x = mean; SD = standard deviation; SE = standard error).

Figure 3 Therapeutic effect on Pgp activity in 10 randomly selected H pylori positive patients: significantly increased post-therapeutic RMF values.

Figure 4 RMF values with five therapeutic protocols: combined therapy had no effect with enhanced Pgp activity. First course: PAM (pantoprazole +amoxicillin+metronidazole), PAC (pantoprazole+amoxicillin+clarithromycin); second course: PAM+PAC, PAM+PAAZ (pantoprazole+amoxicillin+azithromycin), PAC+PAAZ.