Anti-HBV activity of TRL mediated by recombinant adenovirus

Figure 1 EcoRI/HindIII restriction analysis of shuttle plasmid pDC316. M: DNA markers (200 bp DNA ladder); 1: pDC316/TRL; 2: pDC316/TR; 3: pDC316/TRmut; 4: pDC316hEDN; 5: pDC316/HBVc.

Figure 2 The cytopathic effect of HEK293 cells. A: normal HEK293 cells; B: CPE of HEK293.

Figure 3 Evaluation of recombinant adenoviruses by PCR. M: DNA marker (200 bp DNA ladder). 1-5 is the targeted sequence TRL, TR, TRmut, hEDN, HBVc respectively; 6: negative control of RAd.

Figure 4 Expression of TRL in HepG2. 2.15 cells infected by RAd/TRL. A-D: 24, 36, 48, 72 h post-infection, respectively.

Figure 5 Comparison of quantitation of HBV-DNA among groups.