Effects of transforming growth interacting factor on biological behaviors of gastric carcinoma cells

Figure 1 Expression of TGIF protein by cell immunohistochemistry. A: cells stably transfected with pcDNA3.1-TGIF; B: cells stably transfected with PcDNA3.1; C: parental cells.

Figure 2 Expression of TGIF protein by Western blotting. Lanes 1 and 2: TGIF expressing cells; lane 3: vector control cells, lane 4: parental cells. The lower panel was stained with Ponceau S as a loading control.

Figure 3 Proliferation rate of TGIF transfected, vector control and parental cells. A: Without 10 μg/L TGF-1; B: with 10 μg/L TGF-β 1.

Figure 4 Plating efficiency in parental, vector control and TGIF transfected cells.

Figure 5 Morphology of blank, negative control and TGIF transfectant cells by TEM×15000. A: parental cell; B: vector control cell; C: TGIF transfectant cell.

Figure 6 Tumor development in nude mice. A: mice inoculated with parental cells; B: mice inoculated with vector control cells; C: mice inoculated with TGIF expressing cells.