Role of nucleostemin in growth regulation of gastric cancer, liver cancer and other malignancies

Figure 1 Molecular cloning of nucleostemin cDNA fragment. 1, DNA marker: λDNA, EcoRI + Hind III; 2, pGEM-T-NS, BamHI + Hind III.

Figure 2 Detection of NS expression in various tumor tissues and normal human adult kidney tissues using RT-PCR. 1, GAPDH cDNA-fragment sample; 2, NS-cDNA fragment sample; 3, Renal carcinoma tissue; 4, Normal human adult kid-ney tissue; 5, Bladder carcinoma tissue; 6, Liver cancer tissue; 7, Gastric adenocarcinoma tissue; 8, DNA marker: DNA, EcoR I + Hind III.

Figure 3 Detection of NS expression in various tumor tissues and normal human adult kidney tissues by Slot blot. 1, Liver cancer tissue; 2, Bladder carcinoma tissue; 3, Pancreatic cancer tissue; 4, Cardia adenocarcinoma tissue; 5, Gastric adenocarcinoma tissue; 6, Esophagus squamous carcinoma tissue; 7, Denatured NS-cDNA sample; 8, Ovary serous cystadenocarcinoma; 9, Ampulle carci-noma tissue; 10, Renal carcinoma tissue; 11, Normal human adult kidney tissue; 12, Benign hyperplasia tissue of prostate.

Figure 4 Detection of NS expression in various tumor tissues and normal human adult kidney tissues by Northern blot. 1, Gastric adenocarcinoma tissue; 2, Renal carcinoma tissue; 3, Normal human adult kidney tissue; 4, Liver cancer tissue; 5, Bladder carcinoma tissue.

Figure 5 In situ hybridization detection of nucleostemin mRNA in invasive ductal breast carcinoma and normal mammary gland tissue by SP-HRP method. Counterstained with hematoxylin. A: Ductal carcinoma cells showed strong staining in situ (arrows); B: Normal mammary glandular epithelial cells showed no posi-tive staining, only with azury nuclear counterstaining (arrows).