Expression of p21WAF1 and p53 and polymorphism of p21WAF1 gene in gastric carcinoma

Figure 2 PCR amplified products at exon 2 of p21^WAF1 gene. Lines 2, 4, 6, 8, 10: GC. Lines 1, 3, 5, 7, 9: tumor adjacent tissue. M: PCR marker.

Figure 3 RFLP analysis of exon 2 of p21^WAF1 gene and ALW261 digestion analysis of codon 31. Digestion of a 536 bp polymerase chain reaction fragment containing wild type exon 2 yielded fragments of 124, 174, and 238 bp in length (lanes 2, 3). The CA polymerase polymorphism created an extra recognition site resulting in DNA fragments of 76, 98, 124, 174, and 238 bp in the heterozygote 9 (Lanes 4, 5). M: PCR marker. Lane 1: undigested fragment.