Survivin antisense oligodeoxynucleotide inhibits growth of gastric cancer cells

Figure 1 Cytotoxic effect of ASODN targeting different regions of survivin mRNA. Cells were cultured in plastic 96-well plates and quantitated using MTT assay as described in “Materials and Methods.”

Figure 2 Cytotoxic effect of ASODN3 and control sequences.

Figure 3 Survivin and β-actin (loading control) mRNA ex-pression following incubation with antisense, sense,scramble and no ODN control. It shows ethidium bromide staining of conventional agarose gel electrophoresis after amplification.

Figure 4 Survivin protein expression following incubation with surviivn, antisense, sense, scramble and no ODN control. Strong decrease of survivin protein could be detected in the ASODN group.