Protein kinase C-dependent activation of P44/42 mitogen-activated protein kinase and heat shock protein 70 in signal transduction during hepatocyte ischemic preconditioning

Figure 1 Changes of cellular structure and ultrastructure after treatment with IR, IP, IR + PMA, IP + CHE, and IP + PD98059. A1-2: Effect of ischemia and reperfusion on hepatocytes, B1-2: Cytoprotective effects of ischemia preconditioning, C1-2: Effects of ischemia preconditioning after stimulation of PKC with PMA, D1-2: Proteetive effect of ischemia preconditioning abolished by inhibition of PKC with chelerythrine, E1-2: Cytoprotective effect of ischemia preconditioning reverted by PD98059

Figure 2 Changes of cellular ultrastructure after treatment with IR, IP, IR + PMA, IP + CHE, and IP + PD98059. A: Effect of hy-poxia reoxygenation on hepatocytes, B: Cytoprotective effects of hypoxic preconditioning, C: Preconditioning effects mim-icked by stimulation of PKC with PMA, D: Preconditioning protection abolished by inhibition of PKC with chelerythrine, E: Cytoprotection of preconditioning reverted by PD98059.

Figure 3 Expression of P44 and P42 MAPKs in rat liver.

Figure 4 Expression of P44 and P42 MAPKs in isolated human hepatocytes.

Figure 5 Expression of HSP70 in rat liver

Figure 6 Changes of cellular ultrastructure after treatment with IR, IP, IR+PMA, IP+CHE, and IP+PD98059. A: Effect of hypoxia reoxygenation on hepatocytes, B: Cytoprotective effects of hypoxic preconditioning, C: Preconditioning effects mimicked by stimulation of PKC with PMA, D: Preconditioning protection abolished by inhibition of PKC with chelerythrine, E: Cytoprotection of preconditioning reverted by PD98059.