Cryopreservation and gel collagen culture of porcine hepatocytes

Figure 1 A: The thawed hepatocytes’ ultrastructure after 4 mo cryopreservation, (TEM, × 4000), B: The morphology of thawed hepatocytes after 4 h culture (PCM, × 200).

Figure 2 A: Morphology of thawed hepatocytes cultivated in mixed gel for 4 h (PCM, × 200), B: Morphology of hepato-cytes cultivated in sandwich configuration for 24 h (PCM, × 200).

Figure 3 Expression of porcine albumin mRNA on culture hepatocytes after RT-PCR. M: DNA markers; lanes 1, 2 and 3 are control groups, mixed gel collagen and sandwich configuration.

Figure 4 Content of AST on hepatocytes culture medium.

Figure 5 Content of urea nitrogen on hepatocytes culture medium.