Induction of tryptase and histamine release from human colon mast cells by IgE dependent or independent mechanisms

Figure 1 Anti-IgE and calcium ionophore induced histamine re-lease from colon mast cells. The values shown are mean ± SEM for four separate experiments. Stimulus or HBSS alone was incubated with cells for 15 min before termination of the reactions. ^aP < 0.05 compared with spontaneous release group (paired Student’s t test).

Figure 2 Anti-IgE and calcium ionophore induced tryptase re-lease from colon mast cells. The values shown are mean ± SEM for four separate experiments. Stimulus or HBSS alone was incubated with cells for 15 min before termination of the reactions. ^aP < 0.05 compared with spontaneous release group (paired Student’s t test).

Figure 3 Time course for anti-IgE (10 µg/ml) induced release of tryptase (─■─) and histamine (─●─) from colon mast cells. Data shown are mean ± SEM of four separate experiments.

Figure 4 Time course for calcium ionophore (1 µg/ml) induced release of tryptase (─■─) and histamine (─●─) from colon mast cells. Data shown are mean ± SEM of four separate experiments.