Effects of dermatan sulfate derivatives on platelet surface P-selectin expression and protein C activity in blood of inflammatory bowel disease patients

Figure 1 ¹H-NMR and ¹³C-MNR spectra of DS and PSDS. A and B: ¹³C-MNR and ¹H-NMR spectra of DS and the chemical shift of major disaccharides in DS; C and D: ¹³C-MNR and ¹H-NMR spectra of PSDS and the chemical shift of the major disaccharides in PSDS.

Figure 2 Fragment size separation of DS-Oligs. The products were separated on a Superdex 30 size exclusion column (26 mm × 1200 mm) at a flow rate of 0.5 mL/min in 0.25 mol/L ammonium bicarbonate. Elution profiles were monitored by carbazole assay. A and B: depolymerized products of DS and PSDS.

Figure 3 Effects of DS derivatives on expression of P-selectin on IP surface. Bars I, II, III, IV and V represent control group, IBD control group, ADP group, DS group and PSDS group, respectively. Groups 4 to 10 represent DS-Oligs from 1 to 7 listed in Table 1. ^bP < 0.01 vs control group; ^aP < 0.05, ^dP < 0.01 vs IBD control group; ^fP < 0.01 vs ADP group (n = 10 for each group).

Figure 4 Effects of DS and its derivatives on APC activity. Bars I and II represent DS group and PSDS group, respectively. Groups 2 to 8 represent DS-Oligs from 1 to 7 listed in Table 1. ^bP < 0.01 vs DS group, ^dP < 0.01 vs PSDS group (n = 10 for each group).