Preparation of human hepatocellular carcinoma-targeted liposome microbubbles and their immunological properties

Figure 1 Light micrograph of liposome microbubbles under a × 200 field of microscope.

Figure 2 Direct agglutination of targeted liposome microbubbles in the presence of rabbit anti-mouse serum.

Figure 3 Immunofluoresce staining of targeted liposome microbubbles with FITC conjugated sheep anti-mouse IgG. Targeted liposome microbubbles were stained in yellow-green under a × 400 field of fluorescence microscope.

Figure 4 Activity assay of free HAb18 and conjugated HAb18 on microbubbles by ELISA.

Figure 5 Light micrograph of the binding of targeted lipo-some microbubbles to SMMC-7721 cells under a × 400 field of microscope. SMMC-7721 cells were surrounded by targeted liposome microbubbles to form rosettes.

Figure 6 Immunofluorescent assay for identification of the binding of targeted liposome microbubbles to SMMC-7721 cells. SMMC-7721 cells were surrounded by the microbubbles and gave out bright yellow-green fluorescence.

Figure 7 Effect of targeted liposome microbubbles on the proliferation of human hepatocytes and SMMC-7721 cells.