Epstein-Barr virus in hepatocellular carcinogenesis

Figure 1 Results of PCR. P, positive control; N, negative control; B, blank control; M, marker of molecular mass. The sizes of the six bands of the marker are 2000 bp, 1000 bp, 750 bp, 500 bp, 250 bp, 100 bp, respectively. A: β -Globulin (Positive: Lanes 2-4, 210 bp), B: LMP 1 (Positive: Lanes 3 and 4, 337 bp), C: BamHI W (Positive: Lanes 1-6, 130 bp), D: X gene (Positive: Lanes 2-4, 232 bp), E: S gene (Positive: Lanes 1-8, 198 bp), F: HCV (Positive: Lane 4, other lanes are nonspecific, 142bp), G: HDV (All are negative, Lane 5 is nonspecific).

Figure 2 Sequencing results.

Figure 3 Results of immunohistochemistry. A: Positive-control of LMP1 from the tonsil tissue. The cytoplasm of lymphocytes showed positive signals. × 400, B: Immunohistochemical staining with anti-LMP1 antibody of an HCC specimen. The positive signals were localized in the cytoplasm and membrane of neoplasm cells. × 100, C: Immunohistochemical staining with anti-LMP1 antibody of an HCC specimen. The positive signals were localized in the cytoplasm and membrane of neoplasm cells. × 400, D: Immunohistochemical staining with anti-LMP1 antibody of an HCC specimen. The positive signals were localized in the nuclei of neoplasm cells. × 400, E: Immunohistochemical staining with anti-LMP1 antibody of an HCC specimen. The positive signals were localized in the interstitial lymphocytes but no clear positive signals were present in the adjacent neoplasm cells. × 400, F: Immunohistochemical staining with anti-HBsAg antibody of an HCC specimen. The positive signals were localized in the cytoplasm of neoplasm cells. × 400, G: Immunohistochemical staining with anti-HBcAg antibody of an HCC specimen. The positive signals were localized in the cytoplasm and nuclei of neoplasm cells. × 400, H: Immunohistochemical staining with anti-HCV antibody of an HCC specimen. The positive signals were localized in the nuclei of neoplasm cells. × 400.