Selection, proliferation and differentiation of bone marrow-derived liver stem cells with a culture system containing cholestatic serum in vitro

Figure 1 Cell colonies 3 d after selection. Polygonal surrounding cells could be seen.

Figure 2 Appearance of hepatocyte-like colony forming units(H-CFU) 12 d after selection. A: H-CFU, undifferentiated round cells in the center, surrounded by polygonal hepatocyte-like cells B: Regular arrangement of surrounding hepatocyte-like cells similar to the cords of hepatocytes.

Figure 3 Ultrastructure of hepatocyte-like cells. 9000 × .

Figure 4 Positive staining of albumin immunohistochemistry 12 d after selection. ABC staining 200 × .

Figure 5 RT-PCR results. M: marker, 1: AFP, 2: CK-18, 3: CYP2b1, 4: HNF-3β, 5: Albumin, 6: TTR, 7: CK-19, 8: HNF-1α

Figure 6 PAS staining of hepatocyte-like differentiated cells. The cells were positive in the cytoplasm (A) 200 × , (B) 400 × .

Figure 7 Urea synthetic function of bone marrow-derived liver stem cells (mean ± SD, n = 4).