Cloning and sequence analysis of gene oipA encoding an outer membrane protein of human Helicobacter pylori

Figure 1 Amplification of human H pylori oipA gene by PCR. Lane 1: DNA markers (10 000, 8 000, 6 000, 5 000, 4 000, 3 500, 3 000, 2500, 2 000, 1 500, 1 000, 750, 500, 250 bp from top to bottom); Lane 2. oipA gene fragment (924 bp).

Figure 2 Construction technologic route of recombinant plasmids.

Figure 3 Dual endonuclease digestion identifications of re-combinant plasmids. Lane 1: DNA markers (10 000, 8 000, 6 000, 5 000, 4 000, 3 500, 3 000, 2500, 2 000, 1500, 1 000, 750, 500, 250 bp from top to bottom). Lane 2: Digestion fragments of recombinant plasmids.