Brief Reports
Copyright ©The Author(s) 2004.
World J Gastroenterol. Nov 1, 2004; 10(21): 3188-3190
Published online Nov 1, 2004. doi: 10.3748/wjg.v10.i21.3188
Figure 1
Figure 1 Identifications of recombination plasmid. Lane 1: Am-plified from pPIC9K; Lane 2: Amplified from pPIC9K-hALR; Lane 3: pPIC9-hALR digested by XhoI + NotI; Lane M: 1 kb DNA ladder; Lane 4: pPIC9K-hALR digested by SacI + SalI (6.3 kb + 3.4 kb); Lane 5: 3.4 kb fragment with hALR.
Figure 2
Figure 2 SDS-PAGE analysis of purified hALR. Lane M: Pro-tein marker; Lanes 1, 2, and 3: Purified hALR.
Figure 3
Figure 3 Western blot analysis of supernatants from GS115 treated with different plasmids. Lane 1: Supernatant from GS115 with pPIC9K; Lane 2: Supernatant from GS115 with pPIC9K- hALR.