Copyright
©The Author(s) 2004.
World J Gastroenterol. Nov 1, 2004; 10(21): 3188-3190
Published online Nov 1, 2004. doi: 10.3748/wjg.v10.i21.3188
Published online Nov 1, 2004. doi: 10.3748/wjg.v10.i21.3188
Figure 1 Identifications of recombination plasmid.
Lane 1: Am-plified from pPIC9K; Lane 2: Amplified from pPIC9K-hALR; Lane 3: pPIC9-hALR digested by XhoI + NotI; Lane M: 1 kb DNA ladder; Lane 4: pPIC9K-hALR digested by SacI + SalI (6.3 kb + 3.4 kb); Lane 5: 3.4 kb fragment with hALR.
Figure 2 SDS-PAGE analysis of purified hALR.
Lane M: Pro-tein marker; Lanes 1, 2, and 3: Purified hALR.
Figure 3 Western blot analysis of supernatants from GS115 treated with different plasmids.
Lane 1: Supernatant from GS115 with pPIC9K; Lane 2: Supernatant from GS115 with pPIC9K- hALR.
-
Citation: Liu Q, Yu HF, Sun H, Ma HF. Expression of human augmenter of liver regeneration in pichia pastoris yeast and its bioactivity
in vitro . World J Gastroenterol 2004; 10(21): 3188-3190 - URL: https://www.wjgnet.com/1007-9327/full/v10/i21/3188.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i21.3188