Copyright
©The Author(s) 2004.
World J Gastroenterol. Sep 15, 2004; 10(18): 2619-2623
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2619
Published online Sep 15, 2004. doi: 10.3748/wjg.v10.i18.2619
Figure 1 VH and VL and scFv fragments in 20 g/L agarose gel electrophoresis with staining of ethdium bromide (EB).
(A) VH and VL fragments, (B) scFv fragment, M: DGL 2000 marker.
Figure 2 Expression and purification of AD09.
A: SDS-PAGE. Lane 1: Markers (Amersham Pharmacia Biotech, Sweden). Lane 2: Product of E. coli HB2151 without scFv gene. Lane 3: Expression of AD09 in E. coli HB2151 with induction of IPTG. Lane 4: Purified AD09 protein. B: Western blot. Lane 1: Markers. Lane 2: Purified AD09 protein.
Figure 3 Sequence of AD09 gene.
- Citation: Xu MY, Xu XH, Chen GZ, Deng XL, Li J, Yu XJ, Chen MZ. Production of a human single-chain variable fragment antibody against esophageal carcinoma. World J Gastroenterol 2004; 10(18): 2619-2623
- URL: https://www.wjgnet.com/1007-9327/full/v10/i18/2619.htm
- DOI: https://dx.doi.org/10.3748/wjg.v10.i18.2619